|Year : 2020 | Volume
| Issue : 1 | Page : 32-41
Spectrum of germline BRCA mutations in hereditary breast and ovarian cancer syndrome in Indian population: A central reference laboratory experience
Pratiksha Chheda, Shailesh Pande, Tavisha Dama, Sushant Vinarkar, Milind Chanekar, Shweta Limaye, Nilesh Shah, Kirti Chadha
Department of Integrated Oncopathology, Metropolis Healthcare Ltd., Mumbai, Maharashtra, India
|Date of Submission||11-Nov-2019|
|Date of Decision||15-Dec-2019|
|Date of Acceptance||20-Jan-2020|
|Date of Web Publication||24-Feb-2020|
Unit No. 409 to 416, 4th Floor, Commercial Building A, Kohinoor City, Near Kohinoor Mall, Kirol Road, Kurla-W, Mumbai - 400 070, Maharashtra
Source of Support: None, Conflict of Interest: None
Introduction: There is a growing need for BRCA1/BRCA2 mutation frequencies among hereditary breast and ovarian carcinoma (HBOC) cases, specifically determined on the grounds of personal and family history profiles in the Indian population. The current study was intended to identify BRCA1/2 mutation frequency and spectrum in Indian women fulfilling the National Comprehensive Cancer Network criteria.
Methods: One hundred and sixty unrelated women were screened for germline variations in BRCA1 and BRCA2 genes by sequencing. The variants were classified as pathogenic or benign on the basis of American College of Medical Genetics (ACMG) guidelines.
Results: Of 160 women screened for BRCA mutations, 51 (31.9%) carried a pathogenic variant in BRCA1 (n = 36) or BRCA2 (n = 15) gene. An increased frequency of mutation was seen in women with a personal history of breast or ovarian cancer (34.5%) in comparison to unaffected family members (25%). A spectrum of 34 different pathogenic variants (20 in BRCA1 and 14 in BRCA2) was identified in 51 cases. This included a novel variant c.3683_3684dup in BRCA1 which was categorized as a pathogenic variant. The variant c.68_69delAG in BRCA1 was identified in 12/51 positive cases and appears to be the hotspot mutation in the Indian population. In addition, 11 different missense variants were identified in 10 of the study participants and were categorized as variants of unknown clinical significance (VUS) based on the ACMG guidelines. Of these, three of the variants (c.389A>C in BRCA1 and c.3179G>A and c.10124G>T in BRCA2) were unreported in the published literature.
Conclusion: The prevalence of pathogenic variants in the Indian HBOC cohort is high and is similar to other Indian studies. The spectrum of BRCA variants is also diverse, and it is very important to correctly classify them as pathogenic, VUS, or benign. We reinforce the importance of complete BRCA gene screening in the index case, followed by genetic counseling and targeted mutation analysis in other at-risk family members.
Keywords: BRCA1, BRCA2, hereditary breast and ovarian cancer, variant of uncertain significance
|How to cite this article:|
Chheda P, Pande S, Dama T, Vinarkar S, Chanekar M, Limaye S, Shah N, Chadha K. Spectrum of germline BRCA mutations in hereditary breast and ovarian cancer syndrome in Indian population: A central reference laboratory experience. Cancer Res Stat Treat 2020;3:32-41
|How to cite this URL:|
Chheda P, Pande S, Dama T, Vinarkar S, Chanekar M, Limaye S, Shah N, Chadha K. Spectrum of germline BRCA mutations in hereditary breast and ovarian cancer syndrome in Indian population: A central reference laboratory experience. Cancer Res Stat Treat [serial online] 2020 [cited 2020 May 25];3:32-41. Available from: http://www.crstonline.com/text.asp?2020/3/1/32/279065
| Introduction|| |
The most common cancer among Indian women is breast cancer with an incidence of 25.8 and mortality of 12.7/100,000 women. Almost 5%–10% of these are likely to have hereditary breast and ovarian cancer (HBOC) syndrome, and a majority of such cases (85%) are known to be associated with loss-of-function variants in breast cancer susceptibility genes, BRCA1 (MIM# 113705) and BRCA2 (MIM# 600185)., Genetic testing in these BRCA1 and BRCA2 genes has thus become a crucial part of clinical decision-making for women with a strong family background of breast and ovarian cancers and is important for preventive counseling. The lifetime risk in BRCA1- and BRCA2-mutation carriers for developing breast cancer is about 45%-80%, while that of ovarian cancer is >40% and >20% for BRCA-1 mutation carriers and BRCA2-mutation carriers respectively.,,,, Besides breast and ovarian cancers, BRCA1/2 mutations have been associated with several cancers, the most common being pancreas, prostate, and melanoma. Poly (ADP-ribose) polymerases, also called PARP inhibitors, are a new class of chemotherapeutic agents that can target cancers with defective DNA-damage repair. The U.S. Food and Drug Administration and European EMA have approved PARP inhibitors, such as olaparib, for the treatment of BRCA1/ 2 mutation-positive ovarian cancers. Recent Phase III trials demonstrated a 3-month progression-free survival improvement with PARP inhibitors in comparison to single-agent chemotherapy in patients with metastatic breast cancer with mutated germline BRCA. PARP inhibitors, however, seem less effective in metastatic breast cancer patients with wild-type BRCA than in those with BRCA-mutated platinum-sensitive recurrent ovarian cancer. This is probably due to biologic heterogeneity and low somatic BRCA mutation rate in breast cancer.
Testing of BRCA1 and BRCA2 variants in high-risk individuals and families is of utmost importance, and it focuses on identification of the most frequent variants – single-base substitutions or deletions or insertions of one or more bases that result either in premature protein chain termination or in loss of function. Such variants that are found throughout the coding regions and at the splice junctions of BRCA1/ 2 account for 10%–50% of the germline variants in HBOC families. Large genomic rearrangements (LGRs) of BRCA1/ 2 have also been detected in HBOC families, though in a smaller proportion (3%–6%)., These LGRs, however, are reported to be present at a higher frequency of 10%–30% in certain populations, such as Dutch, Italian, and Greek.,,,
There is a significant variation in BRCA1/ 2 mutation type and frequency with regard to race/ethnicity, as well as geographical location. Founder mutations with relatively higher frequency have been identified in certain populations, for example, one variant in BRCA2 ( 6174delT) and two variants (185delAG, 5382insC) in BRCA1 are present at a very high frequency (~2%) in the Ashkenazi Jewish population., Similarly, BRCA2 founder mutation, 999delTCAAA has been reported at a carrier frequency of 0.4%–0.6% in Icelandic population. The contribution and prevalence of these high-risk variants in the Indian HBOC families are relatively unexplored barring a few studies.,,,,,,,,, With a view to identify the mutation spectrum and to contribute toward Indian-specific BRCA1/ 2 data, we analyzed 160 breast and/or ovarian cancer cases for variants in both the genes.
BRCA1/ 2 testing was performed by the 'gold standard' Sanger sequencing, which is technologically reliable with simple workflow and is widely available. However, of late, it has been replaced in clinical laboratories by next-generation sequencing (NGS) technology, which offers high-throughput analysis in a cost-effective manner., Multiplex ligation-dependent probe amplification (MLPA) is usually employed for the detection of LGRs.,,
| Materials and Methods|| |
BRCA1/ 2 mutation screening data of women subjects (cancer affected cases or family members) referred to our laboratory from January 2014 to September 2019 were retrospectively analyzed. All the women underwent pre-test counseling wherein they were informed about the significance and outcome of molecular genetic screening. Detailed personal and family history was obtained, and written informed consent was taken from all the study participants. The study protocol (Project No. MP-01-2018; [Supplementary Appendix 1]: Study Protocol) was approved by 'Conscience Independent Ethics Committee' in November 2018, and the study was conducted in compliance with the principles of the Declaration of Helsinki. The study was not registered in a public clinical trials registry such as 'The Clinical Trials Registry-India.'
Of 291 women who underwent pre-test genetic counseling, 160 unrelated women participants residing across India and fulfilling National Comprehensive Cancer Network (NCCN) criteria (version 3.2019) for genetic testing of HBOC were included in the study [Table 1].
|Table 1: Segregation of study participants with respect to National Comprehensive Cancer Network criteria for genetic risk evaluation|
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Overall, 116 women were affected with cancer (breast cancer n = 73, ovarian cancer n = 43) and 44 women were unaffected at the time of BRCA1/ 2 mutation screening but had ≥1 family member with HBOC. The overall mean age of the 160 screened female patients was 46.3 years (standard deviation [SD], 12.6 years).
BRCA1 and BRCA2 complete gene analysis was carried out by Sanger sequencing for identification of single-nucleotide variations and small insertion–deletions for the initial 86 cases. With technology advancement, the remaining 74 cases were analyzed by NGS. Genomic DNA was extracted from EDTA whole blood by standard procedure using Qiagen QIAmp DNA mini kit.
The coding exons and exon/intron splice junctions of BRCA1/ 2 genes were polymerase chain reaction (PCR) amplified using primer pairs described in BIC database. A total of 78 PCR reactions (32 for BRCA1 and 46 for BRCA2) were set up for each of the patients. This was followed by sequencing using Big Dye Terminator v3.1 Cycle Sequencing Kit on 3500 Dx Genetic Analyzer; Applied Biosystems. To identify the variants, the patient sequences were aligned to the reference sequences (BRCA1 - NM_007294.3; BRCA2 - NM_000059.3) available in NCBI Genbank database using the BioEdit Sequence Alignment software (BioEdit v7.1.11, Ibis Biosciences, Carlsbad, CA).
Genomic DNA was enzymatically fragmented, and regions of interest were selectively enriched using capture probes targeted against coding regions of the BRCA1 and BRCA2 genes, including 10 bp of flanking intronic sequences. Libraries were generated with Illumina compatible adaptors and sequenced on an Illumina platform with minimum coverage of ×20 for every amplicon.
The variants were described as per human genome variation society nomenclature (http://www.hgvs.org/mutnomen) and classified as deleterious (Class 5 – pathogenic or Class 4 – likely pathogenic), variant of unknown clinical significance (VUS, Class 3), likely benign (Class 2), and benign (Class 1) according to the American College of Medical Genetics (ACMG) guidelines. The clinical relevance of the identified variant 'was also indicated in the ClinVar and/or BIC database, National Center for Human Genome Research, NIH, http://research.nhgri.nih.gov/bic/., In addition, in silico analysis tools such as PolyPhen-2 (Polymorphism Phenotyping v2) and MutationTaster were used to predict the deleterious nature of uncommon missense variants.,
The data were analyzed using IBM SPSS Statistics software v. 21.0 (Armonk, NY, USA). Descriptive statistics for continuous variables were presented as “mean ± SD” and for categorical variables as percent frequency. Statistical significance was evaluated for the association between the categorical variables using Chi-square test and Fisher's exact test. P < 0.05 was considered statistically significant.
| Results|| |
A total of 291 women underwent pre-test genetic counseling; 160 women fulfilled the NCCN criteria and were included in the retrospective study. Thirty-six women did not want to proceed with genetic testing, and the remaining 95 women were excluded from retrospective data analysis on the basis of NCCN guidelines [Figure 1]. Of 160 women screened for BRCA1/ 2 mutations, 116 were cancer-affected cases of which 73 of them had a personal history of breast cancer and 43 had ovarian cancer. Remaining 44 women were themselves unaffected during genetic screening but had a family history of cancer, suggestive of BRCA mutation screening. Overall, of the 160 women who underwent BRCA1/ 2 mutation screening test by sequencing method, 51 (31.87%) of them showed the presence of deleterious variants (Class 4 and 5) in BRCA1 or BRCA2 genes. Higher positivity was seen for BRCA1 in comparison to BRCA2 ( 36/51, 70.58% vs. 15/51, 29.4%). The prevalence of pathogenic variant was 34.48% (40 cases) when cancer-affected women (n = 116) were taken into consideration, while among cancer-unaffected women with HBOC family history (n = 44), the prevalence was 25.0% (11 cases). Of 116 cancer-affected cases, the prevalence of BRCA1/ 2 pathogenic variants in breast cancer cases (23/73 – 31.50%) was lower than ovarian cancer cases (17/43 – 39.53%). Further, the number of BRCA1-positive cases was comparatively much higher than BRCA2 mutations in ovarian cancer patients (14 - BRCA1, 3 - BRCA2) than in breast cancer cases (14 - BRCA1, 9 - BRCA2); however, the results were not statistically significant (P = 0.142).
Spectrum of BRCA1/2 pathogenic mutations
A spectrum of 34 different pathogenic variants was identified in 51 cases – 20 in BRCA1 and 14 in BRCA2 [Table 2] and [Table 3]. Among 20 BRCA1 variants, 13 frameshift, five nonsense, and two splice site variants were observed. Similarly, in BRCA2, 10 frameshift, one nonsense, one missense, and two splice site pathogenic variants were detected.
[Figure 2] and [Figure 3] show 34 different pathogenic variants identified in BRCA1 and BRCA2 genes, respectively. The most common variant was c.68_69delAG (p. Glu23Valfs*17) in BRCA1 and was identified in 12 cases, followed by c.5074+1G>A mutation in BRCA1 which was found in three of the cases. Variant - c.4183C>T, c.4035delC, c.516delA in BRCA1 and c.8488-1G>A in BRCA2 were identified in two cases each. The remaining pathogenic variants were identified only once each in the study population. We also came across a novel frameshift variant c.3683_3684dup (p. Leu1229Thrfs*7) which has not been previously reported in the literature. This variant which was identified in a case of ovarian cancer was classified as a Class 5 - pathogenic mutation-based on available evidence as per the ACMG guidelines. Further, several common benign polymorphic variants were observed in the study cohort (data not shared).
|Figure 2: Schematic diagram of the positions of the deleterious mutations identified in the BRCA1 gene|
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|Figure 3: Schematic diagram of the positions of the deleterious mutations identified in the BRCA2 gene|
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Variant of uncertain significance identified
Besides deleterious mutations and common benign polymorphisms, a total of 11 different variants of conflicting pathogenicity or VUS (Class 3 variants) were identified in 10 of 160 (6.25%) women. Of these, four distinct VUS were found in BRCA1 and seven in BRCA2 [Table 4]. Three of the patients showed the presence of two VUS each. In addition, two of the variants, c.7505G>A and c.9728C>T, were found in two of the study participants each. All VUS were missense mutations located within the exonic regions, eight of which were previously reported in the ClinVar database. The remaining three were novel variants unreported in the literature -c.389A>C (p. Tyr130Ser) in BRCA1 and c.3179G>A (p. Ser1060Asn) and c.10124G>T (p. Ser3375Iso) in BRCA2. Although Tyr130Ser variant has not been reported earlier, different variations at the same position, c.389A>T, p. Tyr130Phe, and c.389A>G, p. Tyr130Cys, have been reported in the ClinVar database as VUS. In silico analysis using PolyPhen2 and MutationTaster software suggested that all the exonic variants were likely tolerated, except for three of them that were classified as disease-causing (c.389A>C and c.5107T>C in BRCA1 and c.6935A>T in BRCA2). Further, of these 12 cases showing the presence of unknown variants, one case showed co-occurrence of a pathogenic variant. This case showed the presence of p. Gly800Valfs*10 deleterious variant along with two VUS – p. Ser1060Asn (novel variant) and c.10120A>G. This fact suggests lower possibility of these variants to be disease-causing mutations with deleterious function.
| Discussion|| |
Several therapeutic options and risk-reducing strategies are now available for breast and/or ovarian cancer patients and at-risk family members with pathogenic germline variant in BRCA1/2 genes. Additionally, negative findings on targeted mutation analysis in relatives of known carriers reduce anxiety related to cancer risk, and general population screening can be followed thereafter. We investigated BRCA1/BRCA2 mutations in Indian women fulfilling the NCCN criteria for genetic testing of breast and/or ovarian cancer.
The study comprised BRCA1/ 2 gene sequencing to detect point mutations and small insertions/deletions. We observed deleterious mutations in BRCA1/2 in 31.87% (51/160) of cases. Of the 51 BRCA positives, BRCA1 mutation was detected in 70.58% (36/51) cases and BRCA2 in 29.4% (15/51) cases. In addition, higher proportion of positivity was seen in cancer-affected probands (34.48%) in comparison to unaffected probands with HBOC family history (25.0%). Earlier studies on women of Indian origin with early-onset breast cancer have reported positivity in the range of 2.9% to 50% for BRCA1/BRCA2 mutations.,,,,,, Another Indian study tested 14 genes associated with HBOC and identified mutations in 30.1% (n=304) cases, majority (84.9%) of which were detected in the BRCA1/ 2 genes and few (15.1%) were detected in non-BRCA genes. A recent study in the North Indian population has documented mutation frequency of 30.1% (62/206) for the presence of Class 3, 4, and 5 variants. Our study has documented slightly higher frequency (31.87%) of deleterious mutations by sequencing technology alone (MLPA-based large gene rearrangement was not part of the study) in comparison to other Indian studies. Globally, variable frequency of BRCA1/ 2 mutation has been reported ranging from 10% to 50%.,,,, Studies also support the fact that multigene testing by NGS can yield additional genomic information and increases the rate of mutation detection of risk-associated genes in HBOC.,
The prevalence of mutation in BRCA1 ( 70.58%) was much higher than BRCA2 ( 29.4%) in our study as well as several other studies., This frequency of BRCA1 mutations increases even further when standalone ovarian cancer cases are considered. In the present study, ovarian cancer cases showing BRCA1 mutations were almost five times higher than the ones showing BRCA2 mutations (BRCA1 mutations – 14/23, BRCA2 mutations – 3/23). Similar observation was found in previous studies wherein BRCA1 was 4–8 times more common than BRCA2 in ovarian cancer cases.,,
The spectrum of BRCA1/2 mutations is quite diverse as seen from our data as well as other global studies. In the present study, we have identified 34 different variants in 51 subjects [Table 2] and [Table 3]. The most common mutation was c.68_69delAG (popularly known as 185delAG) in BRCA1 seen in 12 cases, c.5074+1G>A in BRCA1 found in three of the cases, and variants such as c.4183C>T, c.4035delC, c.516delA in BRCA1 and c.8488-1G>A in BRCA2 found twice in the study cohort. Both the BRCA1 variants –c.68_69delAG (common mutations found in Ashkenazi Jews) and c.5074+1G>A – have been previously reported at a higher frequency in other Indian studies.,,, There is another Indian study that specifically concentrated on the identification of c.68_69delAG (185delAG) and c.5946delT (6174delT) founder mutations in 231 breast cancer cases from Eastern India and did not find them in any of the subjects. Except for a few common variants such as c.68_69delAG and c.5266dupC (5382insC) which are noted in most of the populations studied, the global spectrum of BRCA1/ 2 variants is quite diverse. Apart from the known founder mutations, other variants identified at relatively higher frequencies in specific ethnic groups/geographic locations can be combined together to form a targeted panel and offered in those specific populations. However, in our population, although certain variations (c.68_69delAG and c.5074+1G>A in BRCA1) were seen in relatively higher proportion, more studies involving larger number of cases are required to come up with an Indian-specific hotspot mutation panel as a first tier testing. In addition, in case of genetic analysis in at-risk individuals where a pathogenic mutation is already identified in the family, targeted or single-site mutation testing by Sanger sequencing rather than BRCA1/2 complete gene analysis reduces the testing cost to one-tenth.
We came across 11 different VUS in 10 of the 160 patients (6.25%) with majority of them in BRCA2 ( 8 variants). The frequency of VUS reported in different studies across the globe varies widely and depends on the population being analyzed. It ranges from 5% to 6% in probands of European ancestry in the USA to about 21% in African-American populations. The possibility and outcome of having a VUS result should be discussed with the patient during genetic counseling session before the patient decides to proceed with germline mutation analysis. This highlights the importance of pre- and post-test genetic counseling by a qualified genetic counselor. Consideration of PARP inhibitor therapy in patients with VUS is also a matter of concern. In a study carried out by Chevrier et al., 11 patients showed the presence of VUS, which were further classified as potentially benign or deleterious based on allele frequency and Protein Variation Effect Analyzer analysis. VUS predicted to be pathogenic showed better response or PFS than the benign variants when the patients were put on PARP inhibitor therapy. However, larger studies are required to validate the same.
| Conclusion|| |
Our findings of BRCA1/2 mutation profiling on selected HBOC cohort of Indian origin shows that the prevalence of pathogenic variants is quite high and is similar to that reported in other Indian and international studies. Although a heterogeneous spectrum of variants in BRCA1 and BRCA2 is noted, the variants c.68_69delAG and c.5074+1G>A appear to be the most common mutations in the Indian population. Identification of BRCA1/2 mutation in carriers will assist in the management and surveillance focusing on reducing the risk of breast and ovarian cancer, genetic counseling, and testing of other at-risk relatives. Knowledge of BRCA1/2 status in newly diagnosed breast cancer patients may influence surgical decision-making.
We thank Ms. Ankita Chaurasia for assistance with data preparation.
Financial support and sponsorship
All the authors were employed by Metropolis Healthcare Ltd.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Malvia S, Bagadi SA, Dubey US, Saxena S. Epidemiology of breast cancer in Indian women. Asia Pac J Clin Oncol 2017;13:289-95.
Darooei M, Poornima S, Salma BU, Iyer GR, Pujar AN, Annapurna S, et al
. Pedigree and BRCA gene analysis in breast cancer patients to identify hereditary breast and ovarian cancer syndrome to prevent morbidity and mortality of disease in Indian population. Tumour Biol 2017;39:1010428317694303.
Singh J, Thota N, Singh S, Padhi S, Mohan P, Deshwal S, et al
. Screening of over 1000 Indian patients with breast and/or ovarian cancer with a multi-gene panel: Prevalence of BRCA1/2 and non-BRCA mutations. Breast Cancer Res Treat 2018;170:189-96.
King MC, Marks JH, Mandell JB, New York Breast Cancer Study Group. Breast and ovarian cancer risks due to inherited mutations in BRCA1 and BRCA2. Science 2003;302:643-6.
Antoniou A, Pharoah PD, Narod S, Risch HA, Eyfjord JE, Hopper JL, et al
. Average risks of breast and ovarian cancer associated with BRCA1 or BRCA2 mutations detected in case series unselected for family history: A combined analysis of 22 studies. Am J Hum Genet 2003;72:1117-30.
van der Kolk DM, de Bock GH, Leegte BK, Schaapveld M, Mourits MJ, de Vries J, et al
. Penetrance of breast cancer, ovarian cancer and contralateral breast cancer in BRCA1 and BRCA2 families: High cancer incidence at older age. Breast Cancer Res Treat 2010;124:643-51.
Walsh T, Lee MK, Casadei S, Thornton AM, Stray SM, Pennil C, et al
. Detection of inherited mutations for breast and ovarian cancer using genomic capture and massively parallel sequencing. Proc Natl Acad Sci U S A 2010;107:12629-33.
Malone KE, Daling JR, Doody DR, Hsu L, Bernstein L, Coates RJ, et al
. Prevalence and predictors of BRCA1 and BRCA2 mutations in a population-based study of breast cancer in White and Black American women ages 35 to 64 years. Cancer Res 2006;66:8297-308.
Mersch J, Jackson MA, Park M, Nebgen D, Peterson SK, Singletary C, et al
. Cancers associated with BRCA1 and BRCA2 mutations other than breast and ovarian. Cancer 2015;121:269-75.
Dziadkowiec KN, Gąsiorowska E, Nowak-Markwitz E, Jankowska A. PARP inhibitors: Review of mechanisms of action and BRCA1/2 mutation targeting. Prz Menopauzalny 2016;15:215-9.
Zimmer AS, Gillard M, Lipkowitz S, Lee JM. Update on PARP inhibitors in breast cancer. Curr Treat Options Oncol 2018;19:21.
Ewald IP, Ribeiro PL, Palmero EI, Cossio SL, Giugliani R, Ashton-Prolla P. Genomic rearrangements in BRCA1 and BRCA2: A literature review. Genet Mol Biol 2009;32:437-46.
Weitzel JN, Lagos VI, Herzog JS, Judkins T, Hendrickson B, Ho JS, et al
. Evidence for common ancestral origin of a recurring BRCA1 genomic rearrangement identified in high-risk Hispanic families. Cancer Epidemiol Biomarkers Prev 2007;16:1615-20.
Palma MD, Domchek SM, Stopfer J, Erlichman J, Siegfried JD, Tigges-Cardwell J, et al
. The relative contribution of point mutations and genomic rearrangements in BRCA1 and BRCA2 in high-risk breast cancer families. Cancer Res 2008;68:7006-14.
Apessos A, Papadopoulou E, Metaxa-Mariatou V, Agiannitopoulos K, Markopoulos C, Venizelos V, et al
. Different genomic rearrangements account for 17% of BRCA1/2 mutations in Greece. Cancer Res 2015;75:P1-03-08.
Montagna M, Dalla Palma M, Menin C, Agata S, De Nicolo A, Chieco-Bianchi L, et al
. Genomic rearrangements account for more than one-third of the BRCA1 mutations in Northern Italian breast/ovarian cancer families. Hum Mol Genet 2003;12:1055-61.
Petrij-Bosch A, Peelen T, van Vliet M, van Eijk R, Olmer R, Drüsedau M, et al
. BRCA1 genomic deletions are major founder mutations in Dutch breast cancer patients. Nat Genet 1997;17:341-5.
Hogervorst FB, Nederlof PM, Gille JJ, McElgunn CJ, Grippeling M, Pruntel R, et al
. Large genomic deletions and duplications in the BRCA1 gene identified by a novel quantitative method. Cancer Res 2003;63:1449-53.
Moslehi R, Chu W, Karlan B, Fishman D, Risch H, Fields A, et al
. BRCA1 and BRCA2 mutation analysis of 208 Ashkenazi Jewish women with ovarian cancer. Am J Hum Genet 2000;66:1259-72.
Levy-Lahad E, Catane R, Eisenberg S, Kaufman B, Hornreich G, Lishinsky E, et al
. Founder BRCA1 and BRCA2 mutations in Ashkenazi Jews in Israel: Frequency and differential penetrance in ovarian cancer and in breast-ovarian cancer families. Am J Hum Genet 1997;60:1059-67.
Juwle A, Saranath D. BRCA1/BRCA2 gene mutations/SNPs and BRCA1 haplotypes in early-onset breast cancer patients of Indian ethnicity. Med Oncol 2012;29:3272-81.
Vaidyanathan K, Lakhotia S, Ravishankar HM, Tabassum U, Mukherjee G, Somasundaram K. BRCA1 and BRCA2 germline mutation analysis among Indian women from South India: Identification of four novel mutations and high-frequency occurrence of 185delAG mutation. J Biosci 2009;34:415-22.
Kumar BV, Lakhotia S, Ankathil R, Madhavan J, Jayaprakash PG, Nair MK, et al
. Germline BRCA1 mutation analysis in Indian breast/ovarian cancer families. Cancer Biol Ther 2002;1:18-21.
Saxena S, Szabo CI, Chopin S, Barjhoux L, Sinilnikova O, Lenoir G, et al
. BRCA1 and BRCA2 in Indian breast cancer patients. Hum Mutat 2002;20:473-4.
Saxena S, Chakraborty A, Kaushal M, Kotwal S, Bhatanager D, Mohil RS, et al
. Contribution of germline BRCA1 and BRCA2 sequence alterations to breast cancer in Northern India. BMC Med Genet 2006;7:75.
Sharma S, Rajaram S, Sharma T, Goel N, Agarwal S, Banerjee BD. Role of BRCA1 and BRCA2 gene mutations in epithelial ovarian cancer in Indian population: A pilot study. Int J Biochem Mol Biol 2014;5:1-0.
Rajkumar T, Soumittra N, Nancy NK, Swaminathan R, Sridevi V, Shanta V. BRCA1, BRCA2 and CHEK2 (1100 del C) germline mutations in hereditary breast and ovarian cancer families in South India. Asian Pac J Cancer Prev 2003;4:203-8.
Mehta A, Vasudevan S, Sharma SK, Kumar D, Panigrahi M, Suryavanshi M, et al
. Germline BRCA1 and BRCA2 deleterious mutations and variants of unknown clinical significance associated with breast/ovarian cancer: A report from North India. Cancer Manag Res 2018;10:6505-16.
Shah ND, Shah PS, Panchal YY, Katudia KH, Khatri NB, Ray HS, et al
. Mutation analysis of BRCA1/2 mutations with special reference to polymorphic SNPs in Indian breast cancer patients. Appl Clin Genet 2018;11:59-67.
Wallace AJ. New challenges for BRCA testing: A view from the diagnostic laboratory. Eur J Hum Genet 2016;24 Suppl 1:S10-8.
Apessos A, Agiannitopoulos K, Pepe G, Tsaousis GN, Papadopoulou E, Metaxa-Mariatou V, et al
. Comprehensive BRCA mutation analysis in the Greek population. experience from a single clinical diagnostic center. Cancer Genet 2018;220:1-2.
Enyedi MZ, Jaksa G, Pintér L, Sükösd F, Gyuris Z, Hajdu A, et al
. Simultaneous detection of BRCA mutations and large genomic rearrangements in germline DNA and FFPE tumor samples. Oncotarget 2016;7:61845-59.
Alemar B, Gregório C, Herzog J, Matzenbacher Bittar C, Brinckmann Oliveira Netto C, Artigalas O, et al
. BRCA1 and BRCA2 mutational profile and prevalence in hereditary breast and ovarian cancer (HBOC) probands from Southern Brazil: Are international testing criteria appropriate for this specific population? PLoS One 2017;12:e0187630.
NCCN criteria for genetic testing of breast and/or ovarian cancer, NCCN Guideline Version 3.2019. Available from: https://www.nccn.org
. [Last accessed on 2019 Oct 15].
Richards S, Aziz N, Bale S, Bick D, Das S, Gastier-Foster J, et al
. Standards and guidelines for the interpretation of sequence variants: A joint consensus recommendation of the American College of Medical Genetics and genomics and the association for molecular pathology. Genet Med 2015;17:405-24.
Hedau S, Jain N, Husain SA, Mandal AK, Ray G, Shahid M, et al
. Novel germline mutations in breast cancer susceptibility genes BRCA1, BRCA2 and p53 gene in breast cancer patients from India. Breast Cancer Res Treat 2004;88:177-86.
Valarmathi MT, Sawhney M, Deo SS, Shukla NK, Das SN. Novel germline mutations in the BRCA1 and BRCA2 genes in Indian breast and breast-ovarian cancer families. Hum Mutat 2004;23:205.
Fernandes GC, Michelli RA, Galvão HC, Paula AE, Pereira R, Andrade CE, et al
. Prevalence of BRCA1/BRCA2 mutations in a Brazilian population sample at-risk for hereditary breast cancer and characterization of its genetic ancestry. Oncotarget 2016;7:80465-81.
Engel C, Rhiem K, Hahnen E, Loibl S, Weber KE, Seiler S, et al
. Prevalence of pathogenic BRCA1/2 germline mutations among 802 women with unilateral triple-negative breast cancer without family cancer history. BMC Cancer 2018;18:265.
Hirotsu Y, Nakagomi H, Sakamoto I, Amemiya K, Mochizuki H, Omata M. Detection of BRCA1 and BRCA2 germline mutations in Japanese population using next-generation sequencing. Mol Genet Genomic Med 2015;3:121-9.
Sung PL, Wen KC, Chen YJ, Chao TC, Tsai YF, Tseng LM, et al
. The frequency of cancer predisposition gene mutations in hereditary breast and ovarian cancer patients in Taiwan: From BRCA1/2 to multi-gene panels. PLoS One 2017;12:e0185615.
Gayther SA, Russell P, Harrington P, Antoniou AC, Easton DF, Ponder BA. The contribution of germline BRCA1 and BRCA2 mutations to familial ovarian cancer: No evidence for other ovarian cancer-susceptibility genes. Am J Hum Genet 1999;65:1021-9.
Sekine M, Nagata H, Tsuji S, Hirai Y, Fujimoto S, Hatae M, et al
. Mutational analysis of BRCA1 and BRCA2 and clinicopathologic analysis of ovarian cancer in 82 ovarian cancer families: Two common founder mutations of BRCA1 in Japanese population. Clin Cancer Res 2001;7:3144-50.
Karami F, Mehdipour P. A comprehensive focus on global spectrum of BRCA1 and BRCA2 mutations in breast cancer. Biomed Res Int 2013;2013:928562.
Mannan AU, Singh J, Lakshmikeshava R, Thota N, Singh S, Sowmya TS, et al
. Detection of high frequency of mutations in a breast and/or ovarian cancer cohort: Implications of embracing a multi-gene panel in molecular diagnosis in India. J Hum Genet 2016;61:515-22.
Chakraborty A, Banerjee D, Basak J, Mukhopadhyay A. Absence of 185delAG and 6174delT mutations among breast cancer patients of Eastern India. Asian Pac J Cancer Prev 2015;16:7929-33.
Eccles BK, Copson E, Maishman T, Abraham JE, Eccles DM. Understanding of BRCA VUS genetic results by breast cancer specialists. BMC Cancer 2015;15:936.
Chevrier S, Desmoulins I, Favier L, Ghiringhelli F, Bengrine L, Arnould L, et a
l. Prediction of olaparib sensitivity for variants of unknown significance in homologous repair genes. J Clin Oncol 2019;37 Suppl 15:3108.
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3], [Table 4]